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Sanger sequencing, also known as the “chain termination method”, is a method for determining the nucleotide sequence of DNA. Sanger sequencing can be performed manually or, more commonly, in an automated fashion via sequencing machine. Sanger Sequencing Steps 1. DNA Sequence For Chain Termination PCR 2. Size Separation by Gel Electrophoresis 3. Gel Analysis & Determination of DNA Sequence For detailed information watch the video #CapillarySequencing #SangerMethodOfSequencing
Sanger sequencing also known as the "Chain termination method". It is a method for determining the nucleotide sequence of DNA. The method was developed by two time Nobel Laureate 'Fredrick Sanger' and his colleagues in the 1977, hence the name the Sanger sequencing. Please watch this video for more details. If you understood this video please do like, comment, share and subscribe. Other Videos Link - 🤍 By - Chandrajeet Khunte
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Sanger sequencing is based on chain termination method using dideoxynucleotide. It was developed by Frederick Sanger and his collegues in 1977. It employs in vitro replication of DNA using Klenow fragments and necessary dNTPs with required concentration (very small as compared to dNTP) of ddNTPs. Here, the DNA fragment is denatured to its single strand and a primer (oligonucleotide of known sequence) is synthesized and added. There are copies of multiple fragments in the sample. The ssDNA is added to 4 different tubes which consist of ddATP, ddGTP, ddCTP, and ddTTP dideoxynucleotides respectively. All the tubes consist of necessary ingredients for the replication; i.e. DNA polymerase (Klenow fragment), dNTPs, enzyme regulators (Mg2+ ions), etc. and are available collectively in form of Mastermix. The primer added is radiolabelled so that after the PAGE the bands can be visualized by autoradiography. To understand the process easily, please watch this video. If you liked this video, please do like, share and subscribe. :) Thanks for watching. Related videos: 🙋🏻♀️ 🛎Subscribe: 🤍 🗣 Telegram: 🤍 👩🏻💼LinkedIn Page: 🤍 💁🏻 LinkedIn Profile: 🤍 🥳 Facebook Group: 🤍 👩🏻🎓Follow me on Unacademy: 🤍
CELL SIGNALING 🤍 CSIR NET PREPARATION STRATEGY 🤍 UNIT WISE STRATEGY 🤍 MOST AND LEAST IMPORTANT TOPICS FROM EACH UNIT 🤍 PYQ DETAIL DISCUSSION 🤍 🤍 CELL BIOLOGY LECTURES 🤍 GENETICS LECTURES 🤍 BIOCHEMISTRY LECTURES 🤍 MOLECULAR BIOLOGY LECTURES 🤍 IMMUNOLOGY LECTURES 🤍 DEVELOPMENTAL BIOLOGY LECTURES 🤍 ECOLOGY AND EVOLUTION LECTURES 🤍 ANIMAL PHYSIOLOGY LECTURES 🤍 PLANT PHYSIOLOGY LECTURES 🤍 METHODS IN BIOLOGY 🤍 MICROBIOLOGY 🤍 TOPPER'S TALK 🤍 MH SET 🤍 M.Sc. ENTRANCES 🤍 IITJAM PYQ 🤍 GAT B 🤍 ICMR 🤍 I will upload regular video regarding CSIR net and GATE Life science. I have cleared CSIR net with AIR 24 and Gate Life Science. If you want me to make an video on specific topic, please comment below. If you like the video, do share it with your friends. Bansal Biology Instagram handle 🤍 Bansal Biology Telegram channel 🤍bansalbiology #csirnet #gate #iitjam #dbt #icar #icmr #du #hcu #mu #amu #cucet #tu #tifr #gatb #jrf #lifescience #learning #teaching #practice #detail #solution #iiscbanglore #unit2 #detailanalysis #lastyearpapers
DNA Sequencing MSc zoology 2nd sem Hindi notes
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Manual Sanger Sequencing 🤍 All types of Microscopes 🤍 All types of blood groups 🤍 In this video I have explained the automated way of sanger sequencing. in which capillary electrophoresis is being done to sequence the DNA.This video is about sanger method of dna sequencing also known as chain termination or dideoxy method of dna sequencing. Computer based sanger sequencing Sanger sequencing steps Sanger sequencing capillary electrophoresis
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Sanger sequencing method in hindi - This lecture explains about the Sanger sequencing method in hindi. This video lecture explains the Sanger sequencing principle and steps of DNA sequencing with the help of dideoxy nucleotides. Sanger sequencing method of DNA sequencing is also known as the dideoxy DNA sequencing technique. Position of individual nucleotide sequence can be predicted with the help of the Sanger sequencing method of DNA sequencing. For more information, log on to- 🤍 Get Shomu's Biology DVD set here- 🤍 Download the study materials here- 🤍 Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- 🤍shomusbiology.com/dvd-store Shomu’s Biology assignment services – 🤍shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – 🤍shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – 🤍shomusbiology.com Facebook page- 🤍 Twitter - 🤍 SlideShare- 🤍slideshare.net/shomusbiology Google plus- 🤍 LinkedIn - 🤍 Youtube- 🤍 Thank you for watching the video lecture on Sanger sequencing method in hindi.
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The lecture talks about the DNA sequencing and types of DNA sequencing in Hindi
Maxam–Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1976–1977. This method is based on nucleobase-specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides. DNA Sequencing by SANGER METHOD : 🤍
Report says, the UK government has been closely watching the movement of the sub-lineages of the B.1.617 variant that originated in India- B1617.1, B1617.2, and B.617.3. Experts in genomic sequencing of Covid-19 variants in India have now revealed that cases which show B.1.617.2 are slowly eclipsing almost all other variants, including B.1.1.7, the Kent variant that originated in the UK. But the question is what is this genome sequencing? How could it be useful in fighting Kovid's second wave? And where did India miss it? Know all these answers in this video. Install The Lallantop Android App: 🤍 Follow us on: 🤍 Like The Lallantop on Facebook: 🤍 Follow The Lallantop on Twitter: 🤍 Follow Futkar on YouTube for Short videos: 🤍 Follow Latak on Instagram: 🤍 For advertisements e-mail us at: Ads🤍thelallantop.com Watch Lallantop Documentaries: 🤍 Produced By: The Lallantop Edited By:Mohan kanojia
👉Previous Video :🤍 ✔️📚👉 Watch Full Free Course: 🤍 ✔️📚👉 Grab Notes by Expert Teachers Here: 🤍 ✔️📚👉 Grab Books Made by Expert Teachers Here: 🤍 ✔️📚👉 Get All Subjects Playlists: 🤍 ✔️📚👉 Get Any Class & Subject's Topic Video Here:- Https://forms.pabbly.com/form/share/rnqp-495218 = 📢 Full Playlist Link: 🤍 ✅ In this video, ✔️ Class: 11th ✔️ Subject: Biotechnology ✔️ Chapter: Tools and Technologies: Basic Concepts (Chapter 12) ✔️ Topic Name: DNA Sequencing and DNA Microarray ✔️ Topics Covered In This Video (By Muskan Mam): Explanation of DNA Sequencing and DNA Microarray from Tools Ands Technologies: Basic Concepts, Class 11 Biotechnology Chapter 12 has been discussed in the video. = 00:00 Introduction :DNA Sequencing and DNA Microarray 02:17 DNA Sequencing and DNA Microarray 29:07 Sanger's method of DNA sequencing 31:06 Maxam and Gilber method 33:23 Automated DNA Sequencing 38:46 DNA microarray 43:05 Extraction of mRNA 45:00 Probe labelling 46:02 Hybridisation and Washing 53:32 Website Overview = Why study from Magnet Brains? Magnet Brains is an online education platform that helps gives you NCERT/CBSE curriculum-based free full courses from Kindergarten to Class 12th so that you can perform well in any and all exams you give in your academic career. 👉 Contact us 🤑🤑 ➡️ Connect with us : magnetbrainsbhopal🤍gmail.com ➡️ Website : 🤍 ➡️ Subscribe to us on YouTube: 🤍 ➡️ Subscribe to Magnet Brains Hindi Medium : 🤍 ➡️ Subscribe to Magnet Brains IIT JEE & NEET: 🤍 ➡️ Subscribe to Magnet Brains Banking: 🤍 ➡️Facebook-: 🤍 ➡️Telegram-: 🤍 ➡️Instagram:-🤍 #class11biotechnology #magnetbrains #2022_23 #2023 #biotechnology #dna dna microarray steps, dna microarray pdf, dna microarray advantages and disadvantages, dna microarray notes, application of dna microarray, dna microarray technique, cdna microarray, microarrays, . Disclaimer: "This video is for educational and informational purposes only and is not intended to infringe on any copyrights. If you believe that this video has used any copyrighted material in a way that constitutes copyright infringement, please contact us at contact🤍magnetbrains.com and we will take appropriate action."
Hello Dear students! Welcome to my channel 'Medical Globe by Dr Hadi This is Dr Hadi Gold medalist Lecturer Pharmacy Department University of Baluchistan Quetta Pakistan - About This Video - In this Video lecture You will learn Basic concept of DNA sequencing by dideoxynucleotide method also called Sanger 's method. the components of DNA sequencing, how the chain is terminated by the help of dideoxynucleotide triphophate, how the Gel electrophoresis is read to get the DNA sequence and what is the purpose of DNA sequencing. Related videos you can watch - DNA Replication link 🤍 Nucleic acid detail lecture 🤍 About This Channel - This Channel Provides lectures of all subjects medical field specially of Biochemistry, Pharmacology for MBBS and Pharm-D covering all over Pakistan Universities and colleges and other countries where Urdu or English is common language The Lectures are delivered in highly simple and conceptual level so that every student from low grade to sharp grade can take help from. These Lectures can cover most of the medical competitive Exams like mdcat ,Etea CSS, MBBS Entrance Test etc My Channel in Urdu for FSC classes Dr Hafiz sultan academy #DNAsequencing#sangersmethod#Medicalglobebydrhadi
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Maxam–Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1976–1977. This method is based on nucleobase-specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides. Maxam–Gilbert sequencing was the first widely adopted method for DNA sequencing, and, along with the Sanger dideoxy method, represents the first generation of DNA sequencing methods. Maxam–Gilbert sequencing is no longer in widespread use, having been supplanted by next-generation sequencing methods. By - Chandrajeet Khunte
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UPSC IAS (Pre + Mains) LIVE Foundation 2024 Hindi Batch 4 एडमिशन की अंतिम तिथि 30 अप्रैल 2023 है | बैच का समय संध्या 6:00 बजे | Hurry Enrol Now : 🤍 भूगोल मूलभूत सिद्धांत Amazon : 🤍 Flipkart : 🤍 Indian Polity Book For UPSC Amazon : 🤍 Flipkart : 🤍 Bharat Ka Bhugol- Indian Geography Book In Hindi Amazon : 🤍 Flipkart : 🤍 - Dear Viewers, Recently, The Department of Biotechnology said that the exercise to sequence 10,000 Indian human genomes and create a database under the Centre-backed Genome India Project is about two-thirds complete. About 7,000 Indian genomes have already been sequenced of which 3,000 are available for public access to researchers.
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In this video I have explained the topic #GenomeSequencing in (Hindi) #Ritikastutorial #Benefits of genome Sequencing 👇 🤍 Genomics video in HINDI link is provided here 👇 click on the link Part - 1 🤍 part - 2 🤍 #Genome explained in English 🤍 if you like this video then please Subscribe to my channel and also press the bell icon for more notifications 😊
genome sequencing what is genome sequencing
All About Biology-This video is about DNA Sequencing , its types and disadvantages. The different methods like Maxam and Gilbert method , Chain termination Method and Automated DNA sequencung method has been mentioned in the video. For genetic engineering playlist - 🤍 For Techniques of genetic engineering - 🤍 For Microbiology - 🤍 For Genetics all topics - 🤍 For Fungi all topics - 🤍 For Bryophytes all topics - 🤍 For Pteridophytes all topics - 🤍 For Gymnosperms all topics - 🤍 For Plant physiology - 🤍 For NCERT class XII biology - 🤍 Follow me on FACEBOOK - 🤍
DNA Sequencing||Maxam Gilbert Method| : 🤍 DNA Sequencing ||Sanger Method| : 🤍
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Gel Electrophoresis | Automated DNA Sequencing | Sanger Gene Sequencing | Hindi / Urdu Having trouble in understanding process of gel electrophoresis and automated DNA Sequencing. Do not worry. you are at right place to learn it. Learn each and everything about gel electrophoresis and automated DNA Sequencing. What are these process, how they work and what is their advantages. "Aur wo bhe aap ki apni zuban / boli / bhasha main". Link for PDF Lecture File Download : 🤍 Instagram► 🤍 Facebook ► 🤍 YouTube► 🤍 #LetsCrackMDCAT #LetsCrackNeet #LetsCrackNTS #LetsCrackMCAT #Electrophoresis #GelElectrophoresis #DNAElectrophoresis #SangerGeneSequencing #SangerCoulsonGeneSequencing #AutomatedDNASequencing #drasimlectures #medicallectures #biology #anatomy #medicalscience #medicalurdu #medicalhindi
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